Tppp anticorps (Internal Region)
Aperçu rapide pour Tppp anticorps (Internal Region) (ABIN499122)
Antigène
Voir toutes Tppp AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- Internal Region
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Séquence
- C-DPSKDRAAKR LSLES
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Specificité
- Recognizes Tubulin polymerization-promoting protein (TPPP).
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Réactivité croisée (Details)
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Species reactivity (expected):Porcine.
Species reactivity (tested):Human, Mouse and Rat. -
Purification
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
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Immunogène
- Peptide with sequence from the Internal region of the protein sequence according to NP_008961.1. Genename: TPPP
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Indications d'application
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Peptide ELISA: Limit Dilution: 1/32000. Western blot: 0.001-0.01 μg/mL. Detects a band of approx. 26 kDa in Human Brain (Cerebellum),Mouse and Rat Brain lysates. An additional band of unknown identity was also consistentlyobserved at 16 kDa. This band was successfully blocked by incubation with the immunizingpeptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 0.5 mg/mL
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Buffer
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Tppp (Tubulin Polymerization Promoting Protein (Tppp))
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Autre désignation
- TPPP
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Sujet
- Tubulin Polymerization Promoting Protein (TPPP) promotes in vitro polymerization of tubulin into double-walled tubules and polymorphic aggregates or bundled stabilized microtubules blocks. When overexpressed, TPPP inhibits mitotic spindle assembly and nuclear envelope breakdown, apparently without affecting other cellular events.Synonyms: 25 kDa brain-specific protein, TPPP1, Tubulin polymerization-promoting protein
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ID gène
- 11076
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NCBI Accession
- NP_008961
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UniProt
- O94811
Antigène
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